Anti-ZIP Kinase 抗体 (ab2057)
Key features and details
- Rabbit polyclonal to ZIP Kinase
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZIP Kinase antibody
ZIP Kinase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZIP Kinase -
由来種
Rabbit -
特異性
It has no cross reactivity to DAP kinase. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human ZIP Kinase aa 250-350 (internal sequence). ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
Database link: O43293 -
ポジティブ・コントロール
- HeLa whole cell lysate or Jurkat whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2057の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
Autophosphorylated. Phosphorylated by ROCK1. -
細胞内局在
Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase. - Information by UniProt
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参照データベース
- Entrez Gene: 1613 Human
- Omim: 603289 Human
- SwissProt: O43293 Human
- Unigene: 631844 Human
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別名
- DAP kinase 3 antibody
- DAP like kinase antibody
- DAP-like kinase antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence of Jurkat cells staining ZIP Kinase with ab2057 at 10µg/ml
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Western Blot of HeLa and Jurkat cell lysates labeling ZIP Kinase with Anti-ZIP Kinase antibody (ab2057) at 1µg/ml.
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All lanes : Anti-ZIP Kinase antibody (ab2057) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted? -
ab2057 at 10µg/ml staining of ZIP kinase in Jurkat cell by ICC/IF
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Immunofluorescence of ZIPK in Jurkat cells using ab2057 at 10 ug/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab2057 は 3 報の論文で使用されています。
- Yuan S et al. Tumor-associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway. Oncol Lett 18:1840-1846 (2019). PubMed: 31423252
- Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008). PubMed: 18413714
- Oster B et al. Human herpesvirus 6B induces phosphorylation of p53 in its regulatory domain by a CK2- and p38-independent pathway. J Gen Virol 89:87-96 (2008). PubMed: 18089732