Anti-Zinc finger MIZ domain-containing protein 1 抗体 (ab113294)
Key features and details
- Rabbit polyclonal to Zinc finger MIZ domain-containing protein 1
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Zinc finger MIZ domain-containing protein 1 antibody
Zinc finger MIZ domain-containing protein 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Zinc finger MIZ domain-containing protein 1 -
由来種
Rabbit -
特異性
ab113294 antibody will recognize all three known Zinc finger MIZ domain-containing protein 1 isoforms. It is predicted to not cross-react with other PIAS protein family members or other Human proteins based on sequence homology. -
アプリケーション
適用あり: ICC/IF, WB, ICCmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to 15 amino acids near the N terminal of Human Zinc finger MIZ domain-containing protein 1
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ポジティブ・コントロール
- A562 cell lysate tested by WB.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113294の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 115 kDa.
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ICC |
Use a concentration of 10 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 115 kDa. |
ICC
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation. -
組織特異性
Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes. -
配列類似性
Contains 1 SP-RING-type zinc finger. -
ドメイン
The C-terminal proline-rich domain possesses a significant intrinsic transcriptional activity. This activity is inhibited by the N-terminus in the full-length protein. -
細胞内局在
Nucleus speckle. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 57178 Human
- Omim: 607159 Human
- SwissProt: Q9ULJ6 Human
- Unigene: 193118 Human
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別名
- hZIMP10 antibody
- KIAA1224 antibody
- MIZ antibody
see all
画像
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Immunofluorescence of Zinc finger MIZ domain-containing protein 1 in K562 cells with ab113294 at 20 ug/mL.
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All lanes : Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab113294) at 0.5 µg/ml
Lane 1 : K562 cell lysate without blocking peptide
Lane 2 : K562 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 115 kDa -
Immunocytochemical analysis of K562 cells, staining Zinc finger MIZ domain-containing protein 1 with ab113294 at 10 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab113294 は論文での使用が確認できていません。