Anti-Zinc Alpha 2 Glycoprotein 抗体 (ab117275)
Key features and details
- Rabbit polyclonal to Zinc Alpha 2 Glycoprotein
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Zinc Alpha 2 Glycoprotein antibody
Zinc Alpha 2 Glycoprotein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Zinc Alpha 2 Glycoprotein -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysates: Human Liver; Human Plasma Total Protein; Human Heart; Human Lung; Human Adipose. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: HeLa.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117275の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa). |
ターゲット情報
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機能
Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids. -
組織特異性
Blood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver. -
配列類似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 563 Human
- Entrez Gene: 12007 Mouse
- Entrez Gene: 25294 Rat
- Omim: 194460 Human
- SwissProt: P25311 Human
- SwissProt: Q64726 Mouse
- SwissProt: Q63678 Rat
- Unigene: 546239 Human
see all -
別名
- Alpha 2 glycoprotein 1 zinc antibody
- Alpha 2 glycoprotein 1 zinc binding antibody
- Alpha 2 glycoprotein zinc antibody
see all
画像
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ab117275 staining Zinc Alpha 2 Glycoprotein in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab117275 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human Plasma Total Protein Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Lung (Human) Tissue Lysate
Lane 5 : Human adipose normal tissue lysate - total protein (ab28980)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
Zinc Alpha 2 Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
IHC image of ab117275 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117275, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab117275 は 9 報の論文で使用されています。
- Fan G et al. Zinc-a2-glycoprotein promotes browning of white adipose tissue in cold-exposed male mice. Mol Cell Endocrinol 501:110669 (2020). PubMed: 31790716
- Lin B et al. Long non-coding RNA00844 inhibits MAPK signaling to suppress the progression of hepatocellular carcinoma by targeting AZGP1. Ann Transl Med 8:1365 (2020). PubMed: 33313110
- Guo J et al. Adipokine zinc-a2-glycoprotein alleviates lipopolysaccharide-induced inflammatory responses through the ß3-AR/PKA/CREB pathway. Cytokine 123:154742 (2019). PubMed: 31260855
- Liu J et al. AZGP1 inhibits soft tissue sarcoma cells invasion and migration. BMC Cancer 18:89 (2018). WB, IHC-P ; Human . PubMed: 29357838
- Gao SX et al. ZAG alleviates HFD-induced insulin resistance accompanied with decreased lipid depot in skeletal muscle in mice. J Lipid Res 59:2277-2286 (2018). PubMed: 30318474
- Philley JV et al. Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease. Oncotarget 8:18070-18081 (2017). PubMed: 28160560
- Xue Y et al. Zinc-a-2-glycoprotein: a candidate biomarker for colon cancer diagnosis in Chinese population. Int J Mol Sci 16:691-703 (2015). IHC ; Human . PubMed: 25561225
- Priyadarsini S et al. Gross cystic disease fluid protein-15/prolactin-inducible protein as a biomarker for keratoconus disease. PLoS One 9:e113310 (2014). WB ; Human . PubMed: 25405607
- Yang MH et al. Proteomic profiling for peritoneal dialysate: differential protein expression in diabetes mellitus. Biomed Res Int 2013:642964 (2013). PubMed: 23781506