製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ZHX3
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 906-956 of Human ZHX3 (NP_055850.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
IP Use a concentration of 2 - 5 µg/ml.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Acts as a transcriptional repressor. Is a regulator of podocyte gene expression during primary glomerula disease.
  • 組織特異性
    Widely expressed. High expression in kidney.
  • 配列類似性
    Belongs to the ZHX family.
    Contains 2 C2H2-type zinc fingers.
    Contains 5 homeobox DNA-binding domains.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0395 antibody
    • TIX1 antibody
    • Triple homeobox 1 antibody
    • Triple homeobox protein 1 antibody
    • ZHX 3 antibody
    • Zhx3 antibody
    • ZHX3_HUMAN antibody
    • Zinc finger and homeodomain protein 3 antibody
    • Zinc fingers and homeoboxes protein 3 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZHX3 with ab99353 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-ZHX3 antibody (ab99353) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 105 kDa


    Exposure time : 30 seconds
  • Detection of Human ZHX3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab99353 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate. ZHX3 was also immunoprecipitated by another rabbit anti-ZHX3 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 3 seconds.

参考文献

ab99353 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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