Anti-ZHX3 抗体 (ab99353)
Key features and details
- Rabbit polyclonal to ZHX3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZHX3 antibody
ZHX3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZHX3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 906-956 of Human ZHX3 (NP_055850.1)
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ポジティブ・コントロール
- HeLa and 293T whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99353の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
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IP |
Use a concentration of 2 - 5 µg/ml.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 105 kDa. |
IP
Use a concentration of 2 - 5 µg/ml. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acts as a transcriptional repressor. Is a regulator of podocyte gene expression during primary glomerula disease. -
組織特異性
Widely expressed. High expression in kidney. -
配列類似性
Belongs to the ZHX family.
Contains 2 C2H2-type zinc fingers.
Contains 5 homeobox DNA-binding domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 23051 Human
- Omim: 609598 Human
- SwissProt: Q9H4I2 Human
- Unigene: 380133 Human
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別名
- KIAA0395 antibody
- TIX1 antibody
- Triple homeobox 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZHX3 with ab99353 at 1/1000 (0.2µg/ml). Detection: DAB.
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All lanes : Anti-ZHX3 antibody (ab99353) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds -
Detection of Human ZHX3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab99353 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate. ZHX3 was also immunoprecipitated by another rabbit anti-ZHX3 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab99353 は 1 報の論文で使用されています。
- Mondal S et al. PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Nat Methods 19:959-968 (2022). PubMed: 35927480