Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZHX3 with ab99350 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - ZHX3 antibody (ab99350)
All lanes : Anti-ZHX3 antibody (ab99350) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 105 kDa
Exposure time : 30 seconds
Immunoprecipitation - ZHX3 antibody (ab99350)
Detection of Human ZHX3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. ab99350 used for WB at 1 ug/ml and used for IP at 3 ug/mg lysate (lane 1). ZHX3 was also immunoprecipitated by another rabbit anti-ZHX3 antibody (lane 2) and control IgG (lane 3).
Detection Chemiluminescence with exposure times of 10 seconds.