製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ZFP91
  • 特異性
    ab30970 recognises Zinc finger protein 91 homolog (ZFP91)
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog
  • 免疫原

    A region within synthetic peptide: RDETYKPHLE RETPKPRRKS GKVKEEKEKK EIKVEVEVEV KEEENEIRED, corresponding to internal sequence amino acids 231-280 of Human ZFP91

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/312500.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-ZFP91 antibody (ab30970) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate

    Secondary
    HRP conjugated anti-rabbit at 1/10000 dilution

    Predicted band size : 60 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • An J  et al. pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4(CRBN) ubiquitin ligase. Nat Commun 8:15398 (2017). Read more (PubMed: 28530236) »
  • Paschke L  et al. ZFP91: A Noncanonical NF-?B Signaling Pathway Regulator with Oncogenic Properties Is Overexpressed in Prostate Cancer. Biomed Res Int 2016:6963582 (2016). Read more (PubMed: 27975057) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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