The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
The protein encoded by ZFP91 is a member of the zinc finger family of proteins. This protein contains C2H2 type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins.
An J et al. pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4(CRBN) ubiquitin ligase. Nat Commun8:15398 (2017).
Read more (PubMed: 28530236) »
Paschke L et al. ZFP91: A Noncanonical NF-?B Signaling Pathway Regulator with Oncogenic Properties Is Overexpressed in Prostate Cancer. Biomed Res Int2016:6963582 (2016).
Read more (PubMed: 27975057) »