製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Zfp57
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ChIP, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse Zfp57.

    (Peptide available as ab45340.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Wt mouse ES cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration. PubMed: 26728555
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Can be blocked with Mouse Zfp57 peptide (ab45340).

ターゲット情報

  • 関連性
    Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all

画像

  • All lanes : ab45341 at 10 µg

    Lane 1 : Wild-type Mouse ES cell lysate
    Lane 2 : Mouse Zfp57 KO cell lysate
    Lane 3 : Mouse ES-E14TG2a whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa
    Additional bands at: 10 kDa (possible non-specific binding), 149 kDa (possible non-specific binding), 98 kDa (possible non-specific binding)



    Zfp57 KO ES cell lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, University of Cambridge. The mutant mouse used to make these lysates is described here - Takahashi et al., Cold Spring Harb Symp Quant Biol. 2015;80:177-87.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab45341 and anti-GAPDH as a loading control (shown in Red) overnight at 4°C. Antibody binding was detected using ab216773 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

     

プロトコール

参考文献

This product has been referenced in:
  • Anvar Z  et al. ZFP57 recognizes multiple and closely spaced sequence motif variants to maintain repressive epigenetic marks in mouse embryonic stem cells. Nucleic Acids Res 44:1118-32 (2016). ChIP . Read more (PubMed: 26481358) »
  • Riso V  et al. ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells. Nucleic Acids Res 44:8165-78 (2016). Read more (PubMed: 27257070) »

See all 6 Publications for this product

レビューと Q&A

Thank you for contacting us. No, these antibodies have not been tested with overexpression or knockdown cells.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

Our testing did show a band at this range in mouse brain lysate as well (http://www.abcam.com/Zfp57-antibody-ab45341.html#ab45341_1.jpg) that we were not able to identify. To our knowledge, this product has not...

Read More

Thank you for contacting us. Unfortunately it is unlikely that ab45341 works in Human (poor sequence homology). Thank you for sending us the reference of the publication in which ab45341 was used. About ab50944, you mentioned that another lab ...

Read More
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (Testes)
Total protein in input
20 µg
Specification
Testes
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

投稿 Aug 02 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Uterus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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