製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Zfp281
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 775-825 of Human Zfp281 (NP_036614.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab112047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能
    Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GC box binding zinc finger protein 1 antibody
    • GC-box-binding zinc finger protein 1 antibody
    • GZP1 antibody
    • Transcription factor ZBP-99 antibody
    • ZBP 99 antibody
    • ZBP99 antibody
    • zfp281 antibody
    • Zinc finger DNA binding protein 99 antibody
    • Zinc finger DNA-binding protein 99 antibody
    • Zinc finger protein 281 antibody
    • ZN281_HUMAN antibody
    • ZNF281 antibody
    • ZNP 99 antibody
    • ZNP 99 transcription factor antibody
    • ZNP99 antibody
    see all

画像

  • All lanes : Anti-Zfp281 antibody (ab112047) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 97 kDa


    Exposure time : 3 minutes
  • Detection of Zfp281 by Western Blot of Immunprecipitate.
    ab112047 at 1µg/ml staining Zfp281 in HeLa whole cell lysate immunoprecipitated using ab112047 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

参考文献

This product has been referenced in:
  • Li CJ  et al. MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation. J Clin Invest 125:1509-22 (2015). WB ; Mouse . Read more (PubMed: 25751060) »
  • MacArthur BD  et al. Nanog-dependent feedback loops regulate murine embryonic stem cell heterogeneity. Nat Cell Biol 14:1139-47 (2012). WB ; Mouse . Read more (PubMed: 23103910) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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