This antibody gave a positive signal in a wild type mouse embryonic stem cell lysate, which is absent from a Zfp281 null mouse embryonic stem cell lysate.
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Immunoprecipitation - Anti-Zfp281 antibody (ab101318)This image is courtesy of an abreview by Miguel Fidalgo.
Zfp281 was immunoprecipitated using 2µg/ml of ab101318 and 2mg of wild type Mouse embryonic stem cell nuclear extract (lane 1). Mouse embryonic stem cells negative for Zfp281 were used as a negative control (lane 2). Band at ~98kDa is Zfp281.
Fidalgo M et al. Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming. Proc Natl Acad Sci U S A109:16202-7 (2012).
WB, ChIP, IP
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