製品の概要

  • 製品名Anti-Zfp219 antibody
    Zfp219 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Zfp219
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Mouse Zfp219.

    (Peptide available as ab31002.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: F9; IOUD2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

ターゲット情報

Anti-Zfp219 antibody 画像

  • All lanes : Anti-Zfp219 antibody (ab31003) at 1/250 dilution

    Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg/ml per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 58 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-Zfp219 antibody (ab31003) 使用論文

ab31003 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 50 µg
Specification Mouse ES Cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

投稿 Dec 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells & Embryoid Bodies)
Loading amount 40 µg
Specification Mouse Embryonic Stem Cells & Embryoid Bodies
Gel Running Conditions Reduced Denaturing (12% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

投稿 Aug 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"