製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to ZFP161
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    ZFP161 (NP_003400.2, 1 a.a. ~ 449 a.a) full-length human protein.

  • ポジティブ・コントロール
    • HeLa cells, transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml. Note: ab68116 must be purified before use in ICC/IF.
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

ターゲット情報

  • 機能
    Transcriptional repressor of MYC and thymidine kinase promoters (By similarity). Transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'.
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 5 C2H2-type zinc fingers.
  • ドメイン
    The BTB/POZ domain seems to direct the protein to discrete regions in the nucleus.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HZF5 antibody
    • MGC126126 antibody
    • ZBTB14 antibody
    • ZF161_HUMAN antibody
    • ZF5 antibody
    • Zfp 161 antibody
    • Zfp-161 antibody
    • Zfp-5 antibody
    • Zfp161 antibody
    • Zinc finger and BTB domain containing protein 14 antibody
    • Zinc finger and BTB domain-containing protein 14 antibody
    • Zinc finger protein 161 homolog (mouse) antibody
    • Zinc finger protein 161 homolog antibody
    • Zinc finger protein 478 antibody
    • Zinc finger protein 5 antibody
    • Zinc finger protein 5 homolog antibody
    • Zinc finger protein homologous to Zfp161 in mouse antibody
    • ZNF478 antibody
    see all

画像

  • All lanes : Anti-ZFP161 antibody (ab68116) at 1/500 dilution

    Lane 1 : ZFP161 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • HeLa cells were labelled with purified ab68116 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed using 4% paraformaldehyde and permeabilised with 0.1% Triton X-100

参考文献

This product has been referenced in:
  • Maia AT  et al. Effects of BRCA2 cis-regulation in normal breast and cancer risk amongst BRCA2 mutation carriers. Breast Cancer Res 14:R63 (2012). ChIP . Read more (PubMed: 22513257) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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