Anti-Zebrafish Gut Absorptive Cell Epitopes 抗体 [FIS 4E8/1] (ab73643)
Key features and details
- Mouse monoclonal [FIS 4E8/1] to Zebrafish Gut Absorptive Cell Epitopes
- Suitable for: IP, ICC/IF, IHC - Wholemount, WB
- Reacts with: Zebrafish
- Isotype: IgG1
製品の概要
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製品名
Anti-Zebrafish Gut Absorptive Cell Epitopes antibody [FIS 4E8/1] -
製品の詳細
Mouse monoclonal [FIS 4E8/1] to Zebrafish Gut Absorptive Cell Epitopes -
由来種
Mouse -
アプリケーション
適用あり: IP, ICC/IF, IHC - Wholemount, WBmore details -
種交差性
交差種: Zebrafish -
免疫原
Tissue, cells or virus corresponding to Zebrafish Zebrafish Gut Absorptive Cell Epitopes. Lysate of zebrafish intestines.
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特記事項
The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyx at the apical surface of intestinal absorptive cells. This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyx at the apical surface of intestinal absorptive cells. This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells. -
ポリ/モノ
モノクローナル -
クローン名
FIS 4E8/1 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73643の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 24504339
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IHC - Wholemount |
Use at an assay dependent concentration. PubMed: 23352790
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WB |
Use at an assay dependent concentration.
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特記事項 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 24504339 |
IHC - Wholemount
Use at an assay dependent concentration. PubMed: 23352790 |
WB
Use at an assay dependent concentration. |
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab73643 は 5 報の論文で使用されています。
- Levic DS et al. Distinct roles for luminal acidification in apical protein sorting and trafficking in zebrafish. J Cell Biol 219:N/A (2020). IP ; Zebrafish . PubMed: 32328632
- Park J et al. Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut. Dev Cell 51:7-20.e6 (2019). PubMed: 31474562
- Murdoch CC et al. Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization. PLoS Pathog 15:e1007381 (2019). PubMed: 30845179
- Alvers AL et al. Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling. Development 141:1110-9 (2014). ICC/IF ; Zebrafish . PubMed: 24504339
- Crosnier C et al. Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine. Development 132:1093-104 (2005). PubMed: 15689380