製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ZEB1
  • 特異性
    ab81972 is expected to recognize both reported isoforms (NP_001121600.1; NP_110378.3).
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Hamster, Cow, Xenopus laevis, Cynomolgus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human ZEB1 aa 309-323 (internal sequence). According to NP_001121600.1 (or 325-339 of NP_110378.3).
    Sequence:

    C-RPQIRQKIENKPLQE


    Database link: NP_001121600.1

  • ポジティブ・コントロール
    • WB: Human and mouse heart tissue lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.05 - 0.2 µg/ml. Predicted molecular weight: 124 kDa.
ELISA Use at an assay dependent concentration.

Peptide ELISA: antibody detection limit dilution 1/32,000.

ターゲット情報

  • 機能
    Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
  • 組織特異性
    Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
  • 関連疾患
    Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
    Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition.
  • 配列類似性
    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AREB 6 antibody
    • AREB6 antibody
    • BZP antibody
    • Delta crystallin enhancer binding factor 1 antibody
    • DELTA EF1 antibody
    • FECD6 antibody
    • MGC133261 antibody
    • Negative regulator of IL 2 antibody
    • Negative regulator of IL2 antibody
    • NIL 2 A antibody
    • NIL 2 A zinc finger protein antibody
    • NIL 2A antibody
    • NIL-2-A zinc finger protein antibody
    • NIL2A antibody
    • Posterior polymorphous corneal dystrophy 3 antibody
    • PPCD3 antibody
    • Represses interleukin 2 expression antibody
    • TCF 8 antibody
    • TCF-8 antibody
    • TCF8 antibody
    • Transcription factor 8 (represses interleukin 2 expression) antibody
    • Transcription factor 8 antibody
    • ZEB 1 antibody
    • ZEB antibody
    • ZEB1 antibody
    • ZEB1_HUMAN antibody
    • ZFHEP antibody
    • ZFHX 1A antibody
    • ZFHX1A antibody
    • Zinc finger E box binding homeobox 1 antibody
    • Zinc finger E-box-binding homeobox 1 antibody
    • Zinc finger homeodomain enhancer binding protein antibody
    see all

画像

  • Anti-ZEB1 antibody (ab81972) at 0.05 µg/ml + Mouse heart tissue lysate in ripa buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 124 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

プロトコール

参考文献

This product has been referenced in:

See 1 Publication for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic Stem cell)
Permeabilization
Yes - 0.1% triton-x
Specification
Embryonic Stem cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Fixative
1:1 methanol:acetone
Username

Abcam user community

Verified customer

投稿 Dec 17 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic stem cell)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
200000 cells
Specification
embryonic stem cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

投稿 Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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