製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ZDHHC8
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-QRDHPQLKTPPSK

    , corresponding to internal sequence amino acids 708-720 of Human ZDHHC8

  • ポジティブ・コントロール
    • Human Brain (Frontal Cortex) lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Peptide ELISA: antibody detection limit dilution 1:16,000.

    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 81 kDa). An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Palmitoyltransferase involved in glutamatergic transmission. Mediates palmitoylation of ABCA1.
    • 配列類似性
      Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.
      Contains 1 DHHC-type zinc finger.
    • ドメイン
      The DHHC domain is required for palmitoyltransferase activity.
    • 細胞内局在
      Cytoplasmic vesicle membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DHHC 8 antibody
      • DHHC domain containing 8 antibody
      • DHHC-8 antibody
      • KIAA1292 antibody
      • Membrane associated DHHC8 zinc finger protein antibody
      • Probable palmitoyltransferase ZDHHC8 antibody
      • ZDHC8_HUMAN antibody
      • ZDHHC8 antibody
      • ZDHHCL1 antibody
      • Zinc finger DHHC domain containing protein 8 antibody
      • Zinc finger DHHC domain-containing protein 8 antibody
      • Zinc finger protein 378 antibody
      • ZNF378 antibody
      see all

    画像

    • All lanes : Anti-ZDHHC8 antibody (ab59483) at 0.1 µg/ml

      Lane 1 : Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer) without blocking with the immunising peptide
      Lane 2 : Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer) with blocking with the immunising peptide


      Predicted band size : 81 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    参考文献

    ab59483 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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