製品の概要

  • 製品名
    Anti-ZDHHC15 antibody
  • 製品の詳細
    Rabbit polyclonal to ZDHHC15
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: VSRNKTTLEA FCTPVFTSGP EKNGFNLGFI KNIQQVFGDK KKFWLIPIGS SPGDGHSFPM RSMNESQNPL LANEETWEDN EDDNQ, corresponding to internal sequence amino acids 238-322 of Human ZDHHC15.

  • ポジティブ・コントロール
    • Human pancreas tissue; Human liver lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

ターゲット情報

  • 機能
    Palmitoyltransferase specific for GAP43 and DLG4/PSD95.
  • 組織特異性
    Expressed in placenta, liver, lung, kidney, heart and brain.
  • 関連疾患
    Defects in ZDHHC15 are the cause of mental retardation X-linked type 91 (MRX91) [MIM:300577]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • 配列類似性
    Belongs to the DHHC palmitoyltransferase family.
    Contains 1 DHHC-type zinc finger.
  • ドメイン
    The DHHC domain is required for palmitoyltransferase activity.
  • 翻訳後修飾
    Autopalmitoylated.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DHHC-15 antibody
    • Palmitoyltransferase ZDHHC15 antibody
    • UNQ1969/PRO4501 antibody
    • ZDH15_HUMAN antibody
    • Zdhhc15 antibody
    • Zinc finger DHHC domain-containing protein 15 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121203 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121203 at 1/5 dilution staining ZDHHC15 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
  • All lanes : Anti-ZDHHC15 antibody (ab121203) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human Liver cell lysate
    Lane 5 : Human tonsil cell lysate

参考文献

ab121203 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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