Anti-DHHC-15 抗体 (ab121203)
Key features and details
- Rabbit polyclonal to DHHC-15
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DHHC-15 antibody -
製品の詳細
Rabbit polyclonal to DHHC-15 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human DHHC-15 aa 238-322 (internal sequence).
Sequence:VSRNKTTLEAFCTPVFTSGPEKNGFNLGFIKNIQQVFGDKKKFWLIPIGS SPGDGHSFPMRSMNESQNPLLANEETWEDNEDDNQ
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ポジティブ・コントロール
- Human pancreas tissue; Human liver lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121203の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/100 - 1/250.
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ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
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特記事項 |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/100 - 1/250. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
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機能
Palmitoyltransferase specific for GAP43 and DLG4/PSD95. -
組織特異性
Expressed in placenta, liver, lung, kidney, heart and brain. -
関連疾患
Defects in ZDHHC15 are the cause of mental retardation X-linked type 91 (MRX91) [MIM:300577]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. -
配列類似性
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger. -
ドメイン
The DHHC domain is required for palmitoyltransferase activity. -
翻訳後修飾
Autopalmitoylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 158866 Human
- Omim: 300576 Human
- SwissProt: Q96MV8 Human
- Unigene: 253211 Human
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別名
- DHHC-15 antibody
- Palmitoyltransferase ZDHHC15 antibody
- UNQ1969/PRO4501 antibody
see all
画像
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121203 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121203 at 1/5 dilution staining DHHC-15 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
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All lanes : Anti-DHHC-15 antibody (ab121203) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human Liver cell lysate
Lane 5 : Human tonsil cell lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab121203 は 1 報の論文で使用されています。
- Shah BS et al. Regulation of dendrite morphology and excitatory synapse formation by zDHHC15. J Cell Sci 132:N/A (2019). PubMed: 31189538