This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. : This antibody gave a positive result in ELISA against the immunizing peptide (ab73368).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Induces an innate immunity to viral infections by preventing the accumulation of viral RNAs in the cytoplasm. Seems to recruit the RNA processing exosome to degrade the target RNAs. Inhibits alphavirus and filovirus replication.
  • 配列類似性Contains 4 C3H1-type zinc fingers.
    Contains 1 PARP catalytic domain.
    Contains 1 WWE domain.
  • ドメインThe second and fourth zinc fingers are involved in binding to specific viral RNAs.
  • 細胞内局在Cytoplasm. Nucleus. Localizes in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADP ribosyltransferase diphtheria toxin like 13 antibody
    • ARTD13 antibody
    • FLB6421 antibody
    • PARP13 antibody
    • ZAP antibody
    • ZC3H2 antibody
    • ZC3HAV 1 antibody
    • Zc3hav1 antibody
    • ZC3HDC 2 antibody
    • ZC3HDC2 antibody
    • ZCCHV_HUMAN antibody
    • Zinc finger antiviral protein antibody
    • Zinc finger CCCH domain containing protein 2 antibody
    • Zinc finger CCCH domain-containing antiviral protein 1 antibody
    • Zinc finger CCCH domain-containing protein 2 antibody
    • Zinc finger CCCH type antiviral 1 antibody
    • Zinc finger CCCH type antiviral protein 1 antibody
    • Zinc finger CCCH-type antiviral protein 1 antibody
    see all

Anti-ZCCHV antibody 画像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • ICC/IF image of ab73369 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab73369, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 100$% methanol fixed (5 mins) HePG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
  • IHC image of ZCCHV staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73369, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-ZCCHV antibody (ab73369) 使用論文

This product has been referenced in:
  • Liu CH  et al. Battle between influenza A virus and a newly identified antiviral activity of the PARP-containing ZAPL protein. Proc Natl Acad Sci U S A 112:14048-53 (2015). Read more (PubMed: 26504237) »
  • Gläsker S  et al. The alternate triad motif of the poly(ADP-ribose) polymerase-like domain of the human zinc finger antiviral protein is essential for its antiviral activity. J Gen Virol 95:816-22 (2014). WB ; Human . Read more (PubMed: 24457973) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"