製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to ZCCHC8
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    ZCCHC8 (NP_060082, 1 a.a. ~ 707 a.a) full-length human protein.

  • ポジティブ・コントロール
    • ZCCHC8 transfected 293T cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab68739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 79 kDa).

ターゲット情報

  • 機能
    May be involved in pre-mRNA splicing.
  • 配列類似性
    Belongs to the ZCCHC8 family.
    Contains 1 CCHC-type zinc finger.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp434E2220 antibody
    • ZCCHC8 antibody
    • ZCHC8_HUMAN antibody
    • Zinc finger CCHC domain containing 8 antibody
    • Zinc finger CCHC domain containing protein 8 antibody
    • Zinc finger CCHC domain-containing protein 8 antibody
    see all

画像

  • All lanes : Anti-ZCCHC8 antibody (ab68739) at 1/500 dilution

    Lane 1 : ZCCHC8 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Falk S  et al. Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors. Nat Commun 7:13573 (2016). WB . Read more (PubMed: 27905398) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
50 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Mar 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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