製品の概要

  • 製品名Anti-ZCCHC6 antibody
  • 製品の詳細
    Mouse polyclonal to ZCCHC6
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human ZCCHC6 protein (aa 1-412 of AAH32456 or Swissprot id Q5VYS8-5).

  • ポジティブ・コントロール
    • Human liver tissue lysate; ZCCHC6 transfected 293T cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報IHC-P: 1/50.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性ZCCHC6 contains 3 CCHC-type zinc fingers.
    • 参照データベース
    • 別名
      • KIAA1711 antibody
      • MGC38585 antibody
      • PAP associated domain containing 6 antibody
      • PAPD6 antibody
      • Zinc finger CCHC domain containing 6 antibody
      see all

    Anti-ZCCHC6 antibody 画像

    • Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 171 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml

      Lane 1 : ZCCHC6 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 171 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    • ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection. 

    Anti-ZCCHC6 antibody (ab76901) 使用論文

    ab76901 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"