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Synthetic peptide: RNPSRKRKKE KKKKKHRDRK SSDSDSSDSE SDTGKRARHT SKDSKAAKKK , corresponding to C terminal amino acids 179-228 of Human ZCCHC17 (NP_057589)
Our Abpromise guarantee covers the use of ab80454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).Can be blocked with ZCCHC17 peptide (ab171367). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
ab80454 has not yet been referenced specifically in any publications.