製品の概要

  • 製品名Anti-ZC3H13 antibody
    ZC3H13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZC3H13
  • アプリケーション適用あり: ICC/IF, IP, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1175 and 1225 of Human ZC3H13 (NP_055885.2)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa, 293T and NIH3T3 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/400.
IP Use at 2 µg/mg of lysate.
WB 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 197 kDa).

ターゲット情報

  • 配列類似性Contains 1 C3H1-type zinc finger.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2600010B19Rik antibody
    • 3110050K21Rik antibody
    • 4930570G11Rik antibody
    • C87618 antibody
    • DKFZp434D1812 antibody
    • FLJ35669 antibody
    • KIAA0853 antibody
    • ZC3H13 antibody
    • ZC3HD_HUMAN antibody
    • Zinc finger CCCH domain-containing protein 13 antibody
    • zinc finger CCCH type containing 13 antibody
    see all

Anti-ZC3H13 antibody 画像

  • All lanes : Anti-ZC3H13 antibody (ab70802) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of ZC3H13 by Western Blot of Immunoprecipitate.
    ab70802 at 1µg/ml staining ZC3H13 in HeLa whole cell lysate previously immunoprecipitated using ab70802 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: chemiluminescence with exposure time of 30 seconds.
  • ab70802 (1/400) staining ZC3H13 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% TritonX100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

Anti-ZC3H13 antibody (ab70802) 使用論文

This product has been referenced in:
  • Horiuchi K  et al. Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J Biol Chem 288:33292-302 (2013). WB, ICC/IF ; Human . Read more (PubMed: 24100041) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

The concentration of ab70802 is 1 mg/ml. Yes, the recommended antibody dilution is 1/2000 - 1/10000. But the antibody can be used at other dilutions as well, dependenton the samples being studied.

As secon...

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Thank you for contacting us.

The following WB protocol has been used with this antibody, togetherwitha 3-8% SDS Precast gel and HeLa whole cell lysate.

Western Blot Protocol

Reagents Needed:

20X Running Buffer <...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
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Dr. Kirk McManus

Verified customer

投稿 Nov 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"