製品の概要
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製品名Anti-ZC3H13 antibody
ZC3H13 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to ZC3H13
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, IP, WBmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residues 1175 and 1225 of Human ZC3H13 (NP_055885.2)
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ポジティブ･コントロール
- Whole cell lysate from HeLa, 293T and NIH3T3 cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab70802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/400. | |
| IP | Use at 2 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 197 kDa). |
ターゲット情報
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配列類似性Contains 1 C3H1-type zinc finger.
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翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
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参照データベース
- Entrez Gene: 23091 Human
- Entrez Gene: 67302 Mouse
- SwissProt: Q5T200 Human
- Unigene: 136102 Human
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別名
- 2600010B19Rik antibody
- 3110050K21Rik antibody
- 4930570G11Rik antibody
see all
画像
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Western blot - ZC3H13 antibody (ab70802)All lanes : Anti-ZC3H13 antibody (ab70802) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 197 kDa
Observed band size: 240 kDa (why is the actual band size different from the predicted?)
Additional bands at: 200 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation - ZC3H13 antibody (ab70802)Detection of ZC3H13 by Western Blot of Immunoprecipitate.
ab70802 at 1µg/ml staining ZC3H13 in HeLa whole cell lysate previously immunoprecipitated using ab70802 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with exposure time of 30 seconds. -
Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)This image is part of an abreview submitted by Dr. Kirk McManus, University of Manitoba/MICB, Canadaab70802 (1/400) staining ZC3H13 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% TritonX100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
プロトコール
参考文献
This product has been referenced in:
- Horiuchi K et al. Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J Biol Chem 288:33292-302 (2013). WB, ICC/IF ; Human . Read more (PubMed: 24100041) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"