製品の概要
- 製品名Anti-ZBTB40 antibody
ZBTB40 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to ZBTB40
- アプリケーション適用あり: IHC-P, WB, IPmore details
- 種交差性交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- 免疫原
Synthetic peptide, corresponding to a region between residues 1140 and 1190 of human ZBTB40 (NP_055685.3)
- ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- バッファーPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8 -
Concentration information loading... - 精製度Immunogen affinity purified
- 特記事項（精製）ab86330 was affinity purified using an epitope specific to ZBTB40 immobilized on solid support.
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
- Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab86330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 138 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
- 機能May be involved in transcriptional regulation.
- 配列類似性Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 12 C2H2-type zinc fingers. - 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
- 細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 9923 Human
- Omim: 612106 Human
- SwissProt: Q9NUA8 Human
- Unigene: 418966 Human
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別名
- BC059177 antibody
- C230087D24 antibody
- Gm571 antibody
see all
Anti-ZBTB40 antibody 画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB40 antibody (ab86330)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZBTB40 with ab86330 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - ZBTB40 antibody (ab86330)All lanes : Anti-ZBTB40 antibody (ab86330) at 0.1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : 293T whole cell lysates at 5 µg
Developed using the ECL technique
Predicted band size : 138 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes -
Immunoprecipitation - ZBTB40 antibody (ab86330)Detection of ZBTB40 by Western Blot of Immunprecipitate.
ab86330, at 1 µg/ml, staining ZBTB40 in HeLa whole cell lysate immunoprecipitated using ab86330 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 minutes.
Anti-ZBTB40 antibody (ab86330) 使用論文
ab86330 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"