製品の概要

  • 製品名Anti-ZBTB38 antibody
    ZBTB38 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZBTB38
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Pig
  • 免疫原

    A region within synthetic peptide: MSLSRDLKDD FHSDTVLSIL NEQRIRGILC DVTIIVEDTK FKAHSNVLAA, corresponding to N terminal amino acids 1-50 of Human ZBTB38

  • ポジティブ・コントロール
    • HepG2 cell lysate and human kidney tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Peptide ELISA: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 135 kDa).

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

Anti-ZBTB38 antibody 画像

  • Anti-ZBTB38 antibody (ab50664) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1:100,000)

    Predicted band size : 135 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  • Ab50664 (4-8ug/ml) staining human ZBTB38 in human kidney tissue by immunohistochemistry using paraffin embedded tissue. Epithelial cells of renal tubule (indicated with arrows) positively labelled.Ab50664 (4-8ug/ml) staining human ZBTB38 in human kidney tissue by immunohistochemistry using paraffin embedded tissue. Epithelial cells of renal tubule (indicated with arrows) positively labelled.

Anti-ZBTB38 antibody (ab50664) 使用論文

ab50664 has not yet been referenced specifically in any publications.

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Thank you for forwrding your images. They will be very helpful for our investigation into the problem with this antibody.

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As we discussed, I'm sending a free of charge replacement vial of ab50664 which should arrive tomorrow on the order ***. Please keep me updated about the re...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"