製品の概要

  • 製品名Zaprinast
  • 製品の詳細
    Specific cGMP phosphodiesterase inhibitor
  • 生理活性の詳細Specific cGMP phosphodiesterase inhibitor, selective for PDE6, 5, 11 and 9 (IC50 values are 0.15 (PDE6), 0.76 (PDE5), 12 (PDE11) and 29 μM (PDE9)). Reputed GPR35 agonist (EC50 values are 16 and 840 nM at rat and human GPR35 receptors, respectively). Important mediator for a variety of cellular processes including vasodilation.
  • 精製度> 98%

製品の特性

  • 体系名3,6-Dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo[4,5-d]pyrimidin-7-one
  • 分子量271.28
  • 構造式Chemical Structure
  • 分子式C13H13N5O2
  • CAS 番号37762-06-4
  • 保存方法Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • 溶解性Soluble in DMSO to 25 mM
  • 使用に関する注意

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES 線形表記CCCOc1ccccc1c1[nH]c(=O)c2nn[nH]c2n1
  • 由来

    Synthetic

  • 研究分野

    関連製品

    アプリケーション

    Our Abpromise guarantee covers the use of ab120940 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Zaprinast 画像

    • ab76165 staining ERK1 + ERK2 (phospho T202 + Y204) in U87MG cells treated with zaprinast (ab120940), by ICC/IF. Increase of ERK1 + ERK2 (phospho T202 + Y204) expression correlates with increased concentration of zaprinast, as described in literature.
      The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab120940 (zaprinast) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76165 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    Zaprinast (ab120940) 使用論文

    This product has been referenced in:
    • Lugnier C Cyclic nucleotide phosphodiesterase (PDE) superfamily: a new target for the development of specific therapeutic agents. Pharmacol Ther 109:366-98 (2006). Read more (PubMed: 16102838) »
    • Taniguchi Y  et al. Zaprinast, a well-known cyclic guanosine monophosphate-specific phosphodiesterase inhibitor, is an agonist for GPR35. FEBS Lett 580:5003-8 (2006). Read more (PubMed: 16934253) »
    • Soderling SH  et al. Identification and characterization of a novel family of cyclic nucleotide phosphodiesterases. J Biol Chem 273:15553-8 (1998). Read more (PubMed: 9624145) »

    See 0 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"