製品の概要

  • 製品名Anti-ZAP70 (phospho Y319) antibody
    ZAP70 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZAP70 (phospho Y319)
  • 特異性This antibody detects endogenous levels of Zap70 only when phosphorylated at tyrosine 319. The antibody may cross react with Syk when phosphorylatd at equivalent sites: Human - Tyr319, Mouse - Tyr318, Rat - Tyr314.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human ZAP70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D).

  • ポジティブ・コントロール
    • Extracts from Jurkat cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was purified from antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ELISA 1/1000.

ターゲット情報

  • 機能Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
  • 組織特異性Expressed in T- and natural killer cells.
  • 関連疾患Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.
  • ドメインThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
  • 翻訳後修飾Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
  • 細胞内局在Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 70 kDa zeta associated protein antibody
    • 70 kDa zeta-associated protein antibody
    • EC 2.7.10.2 antibody
    • FLJ17670 antibody
    • FLJ17679 antibody
    • Selective T cell defect antibody
    • SRK antibody
    • STD antibody
    • Syk related tyrosine kinase antibody
    • Syk-related tyrosine kinase antibody
    • Truncated ZAP kinase antibody
    • Tyrosine protein kinase ZAP70 antibody
    • Tyrosine-protein kinase ZAP-70 antibody
    • TZK antibody
    • ZAP 70 antibody
    • ZAP70 antibody
    • ZAP70_HUMAN antibody
    • Zeta chain associated protein kinase 70kD antibody
    • Zeta chain associated protein kinase 70kDa antibody
    • Zeta chain associated protein kinase 70kDa isoform 1 antibody
    • Zeta chain associated protein kinase 70kDa isoform 2 antibody
    • Zeta chain TCR associated protein kinase 70kD antibody
    • Zeta chain TCR associated protein kinase 70kDa antibody
    see all

Anti-ZAP70 (phospho Y319) antibody 画像

  • All lanes : Anti-ZAP70 (phospho Y319) antibody (ab47289)

    Lane 1 : Jurkat cells
    Lane 2 : Jurkat cells + phospho peptide


    Predicted band size : 70 kDa
    Observed band size : 70 kDa

Anti-ZAP70 (phospho Y319) antibody (ab47289) 使用論文

ab47289 has not yet been referenced specifically in any publications.

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