製品の概要

  • 製品名Anti-ZAP70 (phospho Y292) antibody
    ZAP70 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZAP70 (phospho Y292)
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from a region of human ZAP70 that contains tyrosine 292 (unfortunately, the amino acid sequence is considered to be commercially sensitive)

  • ポジティブ・コントロール
    • Jurkat cells treated with hydrogen peroxide.
  • 特記事項Centrifuge before opening to settle contents.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: Dulbecco's PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ZAP70. The final product is generated by affinity chromatography using a ZAP70-derived peptide that is phosphorylated at threonine 292.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 74.3 kDa.

ターゲット情報

  • 機能Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
  • 組織特異性Expressed in T- and natural killer cells.
  • 関連疾患Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.
  • ドメインThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
  • 翻訳後修飾Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
  • 細胞内局在Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 70 kDa zeta associated protein antibody
    • 70 kDa zeta-associated protein antibody
    • EC 2.7.10.2 antibody
    • FLJ17670 antibody
    • FLJ17679 antibody
    • Selective T cell defect antibody
    • SRK antibody
    • STD antibody
    • Syk related tyrosine kinase antibody
    • Syk-related tyrosine kinase antibody
    • Truncated ZAP kinase antibody
    • Tyrosine protein kinase ZAP70 antibody
    • Tyrosine-protein kinase ZAP-70 antibody
    • TZK antibody
    • ZAP 70 antibody
    • ZAP70 antibody
    • ZAP70_HUMAN antibody
    • Zeta chain associated protein kinase 70kD antibody
    • Zeta chain associated protein kinase 70kDa antibody
    • Zeta chain associated protein kinase 70kDa isoform 1 antibody
    • Zeta chain associated protein kinase 70kDa isoform 2 antibody
    • Zeta chain TCR associated protein kinase 70kD antibody
    • Zeta chain TCR associated protein kinase 70kDa antibody
    see all

Anti-ZAP70 (phospho Y292) antibody 画像



  • Predicted band size : 74.3 kDa


    Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells left untreated (1) or treated with hydrogen peroxide (2-6) were either left untreated (1-5) or treated with lambda phosphatase (6). Lysates were then incubated with ab12868 following prior incubation with: no peptide (1, 6), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine-containing peptide (4) or, the phosphopeptide immunogen (5). The data show that only the peptide corresponding to ZAP70 [phospho Y292] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Anti-ZAP70 (phospho Y292) antibody (ab12868) 使用論文

This product has been referenced in:
  • Haas A  et al. Intracellular Phospho-Flow cytometry reveals novel insights into TCR proximal signaling events. A comparison with Western blot. Cytometry A 73:799-807 (2008). WB ; Human . Read more (PubMed: 18548611) »

See 1 Publication for this product

Product Wall

Thank you again for your patience, I have just heard back from the originator of this antibody. Are you not seeing any signal at all? The originator said that it worked well for them on an endogenous system. Also, ZAP-70 tyrosine 292 is the negativ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab12868. You mentioned that you are using Jurkat cell lysates; did you treat the lysates with hydrogen peroxide? Jurkat cells treated with hydrogen peroxide are ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"