Anti-ZAP70 抗体 [ZAP-03] (ab38871)
Key features and details
- Mouse monoclonal [ZAP-03] to ZAP70
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-ZAP70 antibody [ZAP-03]
ZAP70 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [ZAP-03] to ZAP70 -
由来種
Mouse -
特異性
ab38871 recognises ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells. -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment representing C-terminal part (160 amino acids) of human ZAP-70.
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ポジティブ・コントロール
- WB: HPB-ALL human peripheral blood leukemia T-cell line. Flow Cyt (Intra): Human B-CLL peripheral blood.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
ZAP-03 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use a concentration of 2 - 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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特記事項 |
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Flow Cyt (Intra)
Use a concentration of 2 - 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
ターゲット情報
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機能
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. -
組織特異性
Expressed in T- and natural killer cells. -
関連疾患
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains. -
ドメイン
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. -
翻訳後修飾
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity. -
細胞内局在
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. - Information by UniProt
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参照データベース
- Entrez Gene: 7535 Human
- Omim: 176947 Human
- SwissProt: P43403 Human
- Unigene: 234569 Human
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別名
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
画像
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Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using ab38871 (concentration in sample 9 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood).
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Separation of human CD3 negative ZAP70 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using ab38871 (concentration in sample 9 μg/ml, GAM APC).
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Flow cytometry intracellular staining pattern of human peripheral whole blood using ab38871 (concentration in sample 9 μg/ml, GAM APC).
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Intracellular Flow Cytometry analysis of the human peripheral blood of a patient with B-cell chronic lymphatic leukemia (B-CLL) using antibody ab38871; Dot Plot diagram shows the distribution of lymphocyte populations: T-lymphocytes (green), B-lymphocytes (blue) and NK cells (red).
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Lane 1 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 4 µg/ml
Lane 2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 2 µg/ml
Lane 3 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 1 µg/ml
Lane 4 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lane 5 : ZAP-70 specific antibody 1E7.2 at 1 µg/ml
Lane 6 : Isotypic control PPV-06 (mouse IgG1).
All lanes : HPB-ALL peripheral blood leukemia T-cell line
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Additional bands at: 55 kDa (possible IgG), 55 kDa (possible degradation product)
Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel; Reducing conditions. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products. Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products. -
Lanes 1-2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lanes 3-4 : Isotypic control PPV-06 (mouse IgG1)
Lanes 1 & 3 : HPB-ALL cell line
Lanes 2 & 4 : RAMOS cell line
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line and RAMOS human Burkitt lymphoma cell line; SDS-PAGE; 10% separating gel, 4% stacking gel.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab38871 は論文での使用が確認できていません。