Anti-YY1 抗体 [EPR4652] - Nuclear Loading Control (ab109237)

製品の概要

  • 製品名Anti-YY1 antibody [EPR4652] - Nuclear Loading Control
    YY1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4652] to YY1 - Nuclear Loading Control
  • アプリケーション適用あり: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human YY1 aa 250-350 (internal sequence).

  • ポジティブ・コントロール
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-YY1 antibody (Alexa Fluor® 488) [EPR4652] - Nuclear Loading Control (ab199814)

    Anti-YY1 antibody (Alexa Fluor® 647) [EPR4652] - Nuclear Loading Control (ab199815)

    Anti-YY1 antibody (HRP) [EPR4652] - Nuclear Loading Control (ab199998)

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.
WB 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/30.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/250 - 1/500.

ICC/IF 1/50.

For unpurified use at 1/100 - 1/250.

ターゲット情報

  • 機能Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
  • 配列類似性Belongs to the YY transcription factor family.
    Contains 4 C2H2-type zinc fingers.
  • 細胞内局在Nucleus matrix. Associated with the nuclear matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CF1 antibody
    • Delta antibody
    • Delta transcription factor antibody
    • INO80 complex subunit S antibody
    • INO80S antibody
    • NF E1 antibody
    • NF-E1 antibody
    • NFE1 antibody
    • OTTHUMP00000197459 antibody
    • Transcriptional repressor protein YY1 antibody
    • TYY1_HUMAN antibody
    • UCR motif DNA binding protein antibody
    • UCRBP antibody
    • Yin and yang 1 antibody
    • Yin and Yang 1 protein antibody
    • Yin Yang 1 antibody
    • Ying Yang 1 antibody
    • YY 1 antibody
    • YY 1 transcription factor antibody
    • YY-1 antibody
    • YY1 antibody
    • YY1 transcription factor antibody
    see all

Anti-YY1 antibody [EPR4652] - Nuclear Loading Control 画像

  • ab109237 staining YY1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/30. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/10000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 45 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling YY1 with purified ab109237 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling YY1 with unpurified ab109237 at 1/100.

  • All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/50000 dilution (purified)

    Lane 1 : Y79 cell lysate
    Lane 2 : HuT-78 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 45 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human tonsil tissue labelling YY1 with unpurified ab109237 at 1/250.

  • All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/2000 dilution (purified)

    Lane 1 : Mouse heart
    Lane 2 : Rat heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 45 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labelling YY1 with unpurified ab109237 at 1/250.

  • Immunocytochemistry/Immunofluorescence analysis of HUT-78 cells labelling YY1 with purified ab109237 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/1000 dilution (unpurified)

    Lane 1 : Daudi cell lysate
    Lane 2 : Y-79 cell lysate
    Lane 3 : HuT-78 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 45 kDa
  • ab109237 (purified) at 1/30 immunoprecipitating YY1 in Y79 cells. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) 使用論文

This product has been referenced in:
  • Yamamoto H  et al. Amla Enhances Mitochondrial Spare Respiratory Capacity by Increasing Mitochondrial Biogenesis and Antioxidant Systems in a Murine Skeletal Muscle Cell Line. Oxid Med Cell Longev 2016:1735841 (2016). WB . Read more (PubMed: 27340504) »
  • Zhao H  et al. An intronic variant associated with systemic lupus erythematosus changes the binding affinity of Yinyang1 to downregulate WDFY4. Genes Immun 13:536-42 (2012). WB . Read more (PubMed: 22972472) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Astatotilapia burtoni Tissue lysate - nuclear (Brain nuclear extracts)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 25 µg
Specification Brain nuclear extracts
Blocking step (agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Sebastian Alvarado

Verified customer

投稿 May 04 2016

Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cell lysate)
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Loading amount 80 µg
Specification Jurkat cell lysate
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Aug 03 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Sample Human Tissue sections (human colon)
Specification human colon
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Dec 10 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample Human Tissue sections (kidney)
Specification kidney
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (murine erythroleukemia (MEL cell))
Loading amount 30 µg
Specification murine erythroleukemia (MEL cell)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Min young Kim

Verified customer

投稿 Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"