製品の概要

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent dilution. PubMed: 20106920
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 49.7 kDa). For optimal results, primary antibody incubations should be performed at 4°C.
EMSA Use at an assay dependent concentration. PubMed: 22210889

ターゲット情報

  • 機能Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
  • 配列類似性Belongs to the YY transcription factor family.
    Contains 4 C2H2-type zinc fingers.
  • 細胞内局在Nucleus matrix. Associated with the nuclear matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CF1 antibody
    • Delta antibody
    • Delta transcription factor antibody
    • INO80 complex subunit S antibody
    • INO80S antibody
    • NF E1 antibody
    • NF-E1 antibody
    • NFE1 antibody
    • OTTHUMP00000197459 antibody
    • Transcriptional repressor protein YY1 antibody
    • TYY1_HUMAN antibody
    • UCR motif DNA binding protein antibody
    • UCRBP antibody
    • Yin and yang 1 antibody
    • Yin and Yang 1 protein antibody
    • Yin Yang 1 antibody
    • Ying Yang 1 antibody
    • YY 1 antibody
    • YY 1 transcription factor antibody
    • YY-1 antibody
    • YY1 antibody
    • YY1 transcription factor antibody
    see all

Anti-YY1 antibody - ChIP Grade 画像



  • Predicted band size : 49.7 kDa


    Detection of YY1 by Western blot analysis. YY1 can be detected in nuclear extracts derived from Raji cells using ab12132 (lane 1) at a 1:1000 dilution. The signal corresponding to nuclear YY1 is eliminated by adding the immunizing peptide to the reaction mixture (lane 2).
  • ICC/IF image of ab12132 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12132, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-YY1 antibody - ChIP Grade (ab12132) 使用論文

This product has been referenced in:
  • Landon AL  et al. MNKs act as a regulatory switch for eIF4E1 and eIF4E3 driven mRNA translation in DLBCL. Nat Commun 5:5413 (2014). WB . Read more (PubMed: 25403230) »
  • Kappei D  et al. HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment. EMBO J 32:1681-701 (2013). IP . Read more (PubMed: 23685356) »

See all 11 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - tween-20
Specification HeLa
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Jun 24 2015

Thank you for contacting us.

I am attaching few questions here, please answers these as these will help us to investigate what went wrong with these antibodies;

Sample preparation (X-ChIP/N-ChIP, cross-linking conditions, DNA fragment...

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