製品の概要

  • 製品名Anti-YL1 antibody
    YL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to YL1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Baboon, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 50-100 of Human YL1 (NP_005988.1)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab112055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

Anti-YL1 antibody 画像

  • Immunoprecipitation of YL1. Samples: 1mg of whole cell lysate from HeLa cells(20% of IP loaded). Antibodies: Affinity purified rabbit anti-YL1 antibody (ab112055) was used at 1 µg/ml. Lane 1- YL1 was also immunoprecipitated by rabbit anti-YL1 antibody (ab112055) at 6 µg/mg lysate. Lane 2- YL1 was immunoprecipitated by an rabbit anti-YL1 antibody that recognizes a downstream epitope. 3- Rabbit IgG control.Detection: Chemiluminescence with exposure time of 10 seconds.
  • All lanes : Anti-YL1 antibody (ab112055) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 41 kDa

Anti-YL1 antibody (ab112055) 使用論文

ab112055 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Melanoma)
Gel Running Conditions Reduced Denaturing (15% gel)
Loading amount 3000 cells
Specification Melanoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Nov 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"