製品の概要

  • 製品名Anti-YKT6 antibody
  • 製品の詳細
    Mouse monoclonal to YKT6
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Dog, Human, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment (proprietary-tag) corresponding to Human YKT6 aa 99-198.
    Sequence:

    DEFSKQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDE TKIILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM


    Database link: NP_006546.1

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.
  • 配列類似性Belongs to the synaptobrevin family.
    Contains 1 longin domain.
    Contains 1 v-SNARE coiled-coil homology domain.
  • ドメインThe longin domain regulates palmitoylation and membrane targeting.
  • 翻訳後修飾Palmitoylated; catalyzes its own palmitoylation. Palmitoylation is required for Golgi targeting.
    Farnesylation is required for Golgi targeting.
  • 細胞内局在Cytoplasm > cytosol. Cytoplasmic vesicle membrane. Golgi apparatus membrane. Probably cycles through vesicles between Golgi and endosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • R SNARE antibody
    • SNARE protein Ykt6 antibody
    • Synaptobrevin homolog YKT6 antibody
    • YKT 6 antibody
    • YKT6 antibody
    • YKT6 S. cerevisiae homolog of antibody
    • YKT6 v SNARE homolog antibody
    • YKT6 v SNARE protein antibody
    • YKT6_HUMAN antibody
    see all

Anti-YKT6 antibody 画像

  • Anti-YKT6 antibody (ab77150) at 5 µg/ml + immunogen (GST-tagged 100 aa recombinant fragment) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 22 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-YKT6 antibody (ab77150) 使用論文

ab77150 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (primary neuron cell)
Gel Running Conditions Reduced Denaturing (13%)
Loading amount 50 µg
Specification primary neuron cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Jie Zhang

Verified customer

投稿 Jul 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"