Anti-YIPF6 抗体 (ab80442)

製品の概要

  • 製品名Anti-YIPF6 antibody
  • 製品の詳細
    Rabbit polyclonal to YIPF6
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide: NFMVRLFVVI VMFAWSIVAS TALLADSQPP NRRALAVYPV FLFYFVISWM , corresponding to C terminal amino acids 179-228 of Human YIPF6

  • ポジティブ・コントロール
    • Fetal muscle lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-YIPF6 antibody 画像

  • Anti-YIPF6 antibody (ab80442) at 1 µg/ml + Fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • ab80442 staining YIPF6 in 293 Human embryonic kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% saponin and blocked with 10% serum for 2 hours at 23°C. Samples were incubated with primary antibody (1/500 in 0.5% saponin) for 2 hours at 23°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-YIPF6 antibody (ab80442) 使用論文

ab80442 has not yet been referenced specifically in any publications.

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I just wanted to update you with the last details provided by my lab colleagues regarding the use of this antibody in different cell lines and tissues.

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Thank you for your reply.

We haven’t had any other complaints from customers using this antibody in WB or IHC, so unfortunately I am unable to inform you about its use in other labs.

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293 human embryonic kidney)
Specification 293 human embryonic kidney
Fixative Formaldehyde
Permeabilization Yes - 0,5%(w/v) saponin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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投稿 Jul 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"