製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Yes1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Yes1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; Jurkat; HEK293; Caco2; MCF7.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

ターゲット情報

  • 機能
    Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾
    Autophosphorylated.
  • 細胞内局在
    Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 別名
    • c Yes antibody
    • Cellular Yes 1 protein antibody
    • Cellular Yes1 protein antibody
    • HsT441 antibody
    • P61 YES antibody
    • p61-Yes antibody
    • Proto oncogene tyrosine protein kinase YES antibody
    • Proto-oncogene c-Yes antibody
    • Tyrosine-protein kinase Yes antibody
    • v yes 1 Yamaguchi sarcoma viral oncogene homolog 1 antibody
    • Viral oncogene yes 1 homolog 1 antibody
    • Viral oncogene yes1 homolog 1 antibody
    • Yamaguchi sarcoma oncogene antibody
    • Yes 1 antibody
    • Yes antibody
    • YES proto oncogene 1, Src family tyrosine kinase antibody
    • YES_HUMAN antibody
    • YES1 antibody
    • YES1 proto oncogene, Src family tyrosine kinase antibody
    see all

画像

  • All lanes : Anti-Yes1 antibody (ab106158) at 1 µg/ml (Milk Blocked - 3%)

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 27 kDa,42 kDa,57 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

プロトコール

参考文献

ab106158 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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