Anti-Yersinia pestis F1 antigen 抗体 [YPF19] (ab20833)

製品の概要

  • 製品名
    Anti-Yersinia pestis F1 antigen antibody [YPF19]
  • 製品の詳細
    Mouse monoclonal [YPF19] to Yersinia pestis F1 antigen
  • アプリケーション
    適用あり: ELISA, WB, ICC/IFmore details
  • 種交差性
    Reacts with Y. pestis. Does not react with Y. pseudotuberculosis and Y. enterocolitica. Not yet tested in otehr species.
  • 免疫原

    Purified F1 antigen from Y. pestis vaccine strain EB

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    >95% pure. Purity is tested by electrophoresis
  • ポリ/モノ
    モノクローナル
  • クローン名
    YPF19
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 18 kDa. Dilution optimised using Chromogenic detection.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 関連性
    Yersinia pestis is a gram-negative coccobacillus belonging to the Enterobacteriaceae. Yersinia pestis is primarily a rodent pathogen, with humans being an accidental host when bitten by an infected rat flea.  The flea draws viable Y. pestis organisms into its intestinal tract. Some Y. pestis in the flea are then regurgitated when the flea gets its blood meal thus transferring the infection to a new host. While growing in the flea, Y. pestis loses its capsular layer, which is made of the F1 (or fraction 1) capsule antigen. Most of the organisms are phagocytosed and killed by the polymorphonuclear leukocytes in the human host. A few bacilli are taken up by tissue macrophages. The macrophages are unable to kill Y. pestis and provide a protected environment for the organisms to synthesize their virulence factors.
  • 細胞内局在
    Secreted
  • 別名
    • Caf1 antibody
    • F1 capsule antigen antibody

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Thank you for the details that you have provided and I'm sorry to hear that you are experiencing difficulty with ab20833. The antibody should absolutely detect purified Yersinia pestis F1 protein. I just need to check a few points; do you know that the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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