製品の概要

  • 製品名Anti-Yersinia pestis antibody [YPF19]
  • 製品の詳細
    Mouse monoclonal [YPF19] to Yersinia pestis
  • 特異性This antibody recognises Y.pestis F1 capsular antigen. There is no cross-reactivity with Y.pseudotuberculosis and Y. enterocolitica.
  • アプリケーション適用あり: IHC-Fr, WB, ELISAmore details
  • 免疫原

    Purified F1 antigen from Yersinia pestis vaccine strain EV76.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名YPF19
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性Y. pestis has a number of virulence factors that enable it to survive in humans by facilitating use of host nutrients, causing damage to host cells, and subverting phagocytosis and other host defense mechanisms. Capsular F1 antigen is a main immunochemical component of Y. pestis surface. Its synthesis is determined by pFra plasmid and induced at 370C. F1 is aggregated on the outer membrane of the Y. pestis microbe as an olygomeric protein, forming a granular layer and gradually diffusing into the environment. Capsular polymer consists of multiple similar hydrophobic protein subunits, aggregating at physiological conditions. F1 subunit of the antigen is originally synthesized from 170 amino acids and has a molecular mass 17.6 K, then, after the elimination of the signal peptide during the secretion onto the surface, a protein with molecular mass 15.6 K and isoelectric point 4.1 is formed.
  • 細胞内局在Cell Membrane
  • 別名
    • Y pestis antibody
    • Y. pestis antibody
    • Y.pestis antibody

Anti-Yersinia pestis antibody [YPF19] (ab8275) 使用論文

This product has been referenced in:
  • Ye Z  et al. Caspase-3 mediates the pathogenic effect of Yersinia pestis YopM in liver of C57BL/6 mice and contributes to YopM's function in spleen. PLoS One 9:e110956 (2014). IHC ; Mouse . Read more (PubMed: 25372388) »

See 1 Publication for this product

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I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Unfortunately we have no information concerning this question, because we have not determined that in our laboratory.

This is a very new product, and I'm afriad all the information we have is on the datasheet. The anitbody has not yet been tested in Western Blotting. A standard protocol for WB is available in the "Protocols" section of the Abcam website

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"