Anti-YAP1 (phospho S127) 抗体 [EP1675Y] (ab76252)

製品の概要

• 製品名
  Anti-YAP1 (phospho S127) antibody [EP1675Y]
  YAP1 一次抗体 製品一覧
• 製品の詳細
  Rabbit monoclonal [EP1675Y] to YAP1 (phospho S127)
• 特異性
  ab76252 detects YAP1 phosphorylated at Serine 127.
• アプリケーション
  適用あり: WB, IHC-Pmore details
  適用なし: ICC/IF
• 種交差性
  交差種: Mouse, Rat, Human
  
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human YAP1 (phospho S127).

• ポジティブ･コントロール
  • WB: HeLa, C6 and NIH/3T3 cell lysates treated with FBS + Calyculin A. IHC-P: Mouse kidney, rat stomach and human adenocarcinoma of the endometrium tissues.
• 特記事項

  Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  This product is a recombinant rabbit monoclonal antibody.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
• バッファー
  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
• Concentration information loading...
• 精製度
  Protein A purified
• ポリ/モノ
  モノクローナル
• クローン名
  EP1675Y
• アイソタイプ
  IgG
• 研究分野
  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Src Family

  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Cancer
  • Signal transduction
  • Other

関連製品

• Alternative Versions
  • Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Immunohistochemistry kits
  • EXPOSE Rabbit specific HRP/DAB detection IHC kit (ab80437)
• Positive Controls
  • Kidney (mouse) normal tissue slides (ab4606)

アプリケーション

ターゲット情報

• 機能
  Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
• 組織特異性
  Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
• 配列類似性
  Belongs to the YORKIE family.
  Contains 2 WW domains.
• 翻訳後修飾
  Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
• 細胞内局在
  Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.

• Target information above from: UniProt accession P46937 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 10413 Human
  • Entrez Gene: 22601 Mouse
  • Entrez Gene: 363014 Rat
  • Omim: 606608 Human
  • SwissProt: P46937 Human
  • SwissProt: P46938 Mouse
  • SwissProt: Q2EJA0 Rat
  • Unigene: 503692 Human

  • Unigene: 221992 Mouse
  • Unigene: 162978 Rat

  see all
• 別名
  • 65 kDa Yes associated protein antibody
  • 65 kDa Yes-associated protein antibody
  • COB1 antibody

  • YAp 1 antibody
  • YAP 65 antibody
  • YAP antibody
  • YAP1 antibody
  • YAP1_HUMAN antibody
  • YAP2 antibody
  • YAP65 antibody
  • yes -associated protein delta antibody
  • Yes associated protein 1 65kDa antibody
  • Yes associated protein 1 antibody
  • Yes associated protein 2 antibody
  • yes associated protein beta antibody
  • YKI antibody
  • Yorkie homolog antibody

  see all

プロトコール

• Western blot protocols
• Immunohistochemistry protocols