Anti-YAP1 (phospho S127) 抗体 [EP1675Y] (ab76252)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1675Y] to YAP1 (phospho S127)
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-YAP1 (phospho S127) antibody [EP1675Y]
YAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1675Y] to YAP1 (phospho S127) -
由来種
Rabbit -
特異性
Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
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アプリケーション
適用あり: WB, IHC-P, Dot blotmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, C6 and NIH/3T3 cell lysates treated with Calyculin A. 293A grown in serum-free media. IHC-P: Human endometrium cancer, Mouse kidney, Mouse stomach, Rat kidney and Rat stomach tissues. Dot Blot: YAP1 peptides.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1675Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 - 75 kDa).
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IHC-P |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Dot blot |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 - 75 kDa). |
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3). -
組織特異性
Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). -
配列類似性
Belongs to the YORKIE family.
Contains 2 WW domains. -
翻訳後修飾
Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. -
細胞内局在
Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density. - Information by UniProt
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参照データベース
- Entrez Gene: 10413 Human
- Entrez Gene: 22601 Mouse
- Entrez Gene: 363014 Rat
- Omim: 606608 Human
- SwissProt: P46937 Human
- SwissProt: P46938 Mouse
- SwissProt: Q2EJA0 Rat
- Unigene: 503692 Human
see all -
別名
- 65 kDa Yes associated protein antibody
- 65 kDa Yes-associated protein antibody
- COB1 antibody
see all
画像
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All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2 : HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg
Lane 3 : HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 65 - 75 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded sections of human endometrium cancer tissue labelling YAP1 (S127) with ab76252 at 1/500 (0.428 μg/ml) dilution, followed by ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human endometrium cancer without alkaline phosphatase treatment; No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab76252 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Dot blot analysis of YAP1 peptides labelling YAP1 (pS127) with ab76252 at 1/1000 dilution (0.24μg/ml). ab97051 (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated) was used as the secondary antibody at 1/100000 dilution.
Lane 1: YAP1 non-phospho peptide
Lane 2: YAP1 S127 phospho peptide
Lane 3: YAP1 S128 phospho peptide
Lane 4: YAP1 S131 phospho peptide
Lane 5: YAP1 S127+S128 phospho peptide
Lane 6: YAP1 S127+S131 phospho peptide
Lane 7: YAP1 S128+S131 phospho peptide
Lane 8: YAP1 S127+S128+S131 phospho peptideBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 2 : NIH/3T3 treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg
Lane 3 : NIH/3T3 treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 65 - 75 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking buffer: 5% NFDM/TBST.
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All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/1000 dilution
Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 2 : C6 treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg
Lane 3 : C6 treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 65 - 75 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking buffer: 5% NFDM/TBST
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All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/1000 dilution
Lane 1 : 293A grown in serum-free media overnight, whole cell lysate
Lane 2 : 293A grown in serum-free media overnight, then 10% FBS was added to medium for 1 hour, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 - 75 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded sections of rat kidney tissue labelling YAP1 (S127) with ab76252 at 1/500 (0.428 μg/ml) dilution, followed by ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on rat kidney without alkaline phosphatase treatment; No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab76252 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemical analysis of paraffin-embedded sections of mouse stomach tissue labelling YAP1 (S127) with ab76252 at 1/500 (0.428 μg/ml) dilution, followed by ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on mouse stomach without alkaline phosphatase treatment; No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab76252 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of endometrium tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (79)
ab76252 は 79 報の論文で使用されています。
- Wang X et al. Yes-associated protein reacts differently in vascular smooth muscle cells under different intensities of mechanical stretch. Aging (Albany NY) 14:286-296 (2022). PubMed: 34983026
- Ahmad US et al. Desmoglein-3 induces YAP phosphorylation and inactivation during collective migration of oral carcinoma cells. Mol Oncol 16:1625-1649 (2022). PubMed: 35000271
- Yang L et al. Targeting PLA2G16, a lipid metabolism gene, by Ginsenoside Compound K to suppress the malignant progression of colorectal cancer. J Adv Res 36:265-276 (2022). PubMed: 35127176
- Chen T et al. Effect of e-cigarette refill liquid on follicular development and estrogen secretion in rats. Tob Induc Dis 20:36 (2022). PubMed: 35529323
- Tu J et al. Growth arrest-specific transcript 5 represses endometrial cancer development by promoting antitumor function of tumor-associated macrophages. Cancer Sci 113:2496-2512 (2022). PubMed: 35534987