The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 108 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes.
組織特異性Widely expressed. Expressed at higher level in placenta, kidney and pancreas. Weakly expressed in skeletal muscle.
配列類似性Belongs to the glycosyltransferase 14 family. XylT subfamily.
翻訳後修飾Contains 7 disulfide bonds. N-glycosylated.
細胞内局在Endoplasmic reticulum membrane. Golgi apparatus membrane. Secreted. Some fraction is also found in the extracellular space together with chondroitin sulfate proteoglycans, suggesting that it can be secreted.