製品の概要

  • 製品名Anti-XRCC4 antibody
    XRCC4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to XRCC4
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 266-279 of Human XRCC4 (NP_071801)

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and HeLa cells. A549 xenograft.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the XRCC4 family.
  • 翻訳後修飾Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
    Monoubiquitinated.
    Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
    • DNA repair protein XRCC4 antibody
    • SSMED antibody
    • X ray repair complementing defective repair in Chinese hamster cells 4 antibody
    • X ray repair cross complementing 4 antibody
    • X ray repair cross complementing protein 4 antibody
    • X-ray repair cross-complementing protein 4 antibody
    • XRCC 4 antibody
    • XRCC4 antibody
    • XRCC4_HUMAN antibody
    see all

Anti-XRCC4 antibody 画像

  • All lanes : Anti-XRCC4 antibody (ab97351) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLaS3 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 38 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97351 at 1/200 dilution. Lower image was also labelled with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97351 at 1/100 dilution.

Anti-XRCC4 antibody (ab97351) 使用論文

ab97351 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ChIP
Sample Human Cell lysate - whole cell (U2OS)
Negative control Mock, untreated cells
Specification U2OS
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control AsiSI induced DSB no1 and no2 (positive signal for anti XRCC4 ab145, RAD51 and gamma H2AX)
Username

Dr. Thomas Clouaire

Verified customer

投稿 Dec 21 2015

Thank you very much for your call this morning and for your patience while I've reviewed the literature and other XRCC4 antibodies.

I found antibodies sold by other companies showingeither band size,two of which show bands at both 38 and 55 k...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (VSMC)
Specification VSMC
Fixative Formaldehyde
Permeabilization Yes - NP40
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Andrew Cobb

Verified customer

投稿 Mar 09 2012

Application Western blot
Sample Mouse Tissue lysate - whole (mouse liver lysate)
Loading amount 10 µg
Specification mouse liver lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Oct 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"