製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to XRCC3
  • 特異性
    ab58467 detects endogenous levels of total XRCC1 protein.
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from internal sequence of human XRCC3.

  • ポジティブ・コントロール
    • Extracts from HepG2 cells; human lung carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

ターゲット情報

  • 機能
    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
  • 配列類似性
    Belongs to the RecA family. RAD51 subfamily.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CMM6 antibody
    • DNA repair protein XRCC3 antibody
    • RAD51 like antibody
    • X ray repair complementing defective repair in Chinese hamster antibody
    • X ray repair complementing defective repair in Chinese hamster cells 3 antibody
    • X ray repair cross complementing protein 3 antibody
    • X-ray repair cross-complementing protein 3 antibody
    • XRCC 3 antibody
    • XRCC3 antibody
    • XRCC3_HUMAN antibody
    see all

画像

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab58467 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • All lanes : Anti-XRCC3 antibody (ab58467) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with no immunising peptide
    Lane 2 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with immunising peptide


    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • ab58467 at 1/500 dilution staining XRCC3 in COS7 cells by Immunofluorescence, in the absence or presence of the synthesized peptide.

参考文献

ab58467 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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