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Synthetic peptide (Human) conjugated to KLH - which represented aportion within the last 100 amino acids of the human X-ray Repair Cross Complementing Protein 1 (GenBank PID 11425942).
Our Abpromise guarantee covers the use of ab9147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 - 6 µg/ml.|
|WB||1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa).|
|ICC/IF||1/200 - 1/500.|
ab9147 staining human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-rabbit antibody diluted 1/400 was used as the secondary.