製品の概要

  • 製品名Anti-XRCC1 antibody [33-2-5]
    XRCC1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [33-2-5] to XRCC1
  • 特異性This antibody is specific for XRCC1 (85 kDa).
  • アプリケーション適用あり: WB, IP, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Rat, Human, Chinese Hamster
  • 免疫原

    Recombinant full length protein corresponding to Human XRCC1.

  • ポジティブ・コントロール
    • Testis.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC/IF 1/200. See Abreview.
Flow Cyt Use 1µg for 106 cells.

ターゲット情報

  • 機能Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • 配列類似性Contains 2 BRCT domains.
  • 翻訳後修飾Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
    Sumoylated.
  • 細胞内局在Nucleus. Accumulates at sites of DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA repair protein XRCC1 antibody
    • RCC antibody
    • X ray repair complementing defective repair in chinese hamster antibody
    • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
    • X ray repair complementing defective repair in chinese hamster cells 1 antibody
    • X ray repair cross complementing 1 antibody
    • X ray repair cross complementing protein 1 antibody
    • X ray repair, complementing defective, repair in Chinese hamster antibody
    • X-ray repair cross-complementing protein 1 antibody
    • XRCC 1 antibody
    • Xrcc1 antibody
    • XRCC1_HUMAN antibody
    see all

Anti-XRCC1 antibody [33-2-5] 画像

  • ab1838 - immunohistochemistry

    Formalin fixed paraffin embedded human testis stained with XRCC1, using ABC and AEC chromogen.

  • All lanes : Anti-XRCC1 antibody [33-2-5] (ab1838) at 1/500 dilution

    Lane 1 : Human dermal fibroblast - whole cell lysate. Treated with non-targeting siRNA
    Lane 2 : Human dermal fibroblast - whole cell lysate. Treated with XRCC1 siRNA

    Secondary
    HRP conjugated rabbit ant-mouse antibody at 1/2500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab1838 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1838, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-XRCC1 antibody [33-2-5] (ab1838) 使用論文

This product has been referenced in:
  • Bowen C  et al. NKX3.1 Suppresses TMPRSS2-ERG Gene Rearrangement and Mediates Repair of Androgen Receptor-Induced DNA Damage. Cancer Res 75:2686-98 (2015). Read more (PubMed: 25977336) »
  • Das BB  et al. PARP1-TDP1 coupling for the repair of topoisomerase I-induced DNA damage. Nucleic Acids Res 42:4435-49 (2014). Human . Read more (PubMed: 24493735) »

See all 21 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (mef)
Loading amount 40 µg
Specification mef
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 May 16 2012

Thank you for your reply.

I am very pleased to hear you would like to accept our offer and test ab1838 in mouse. As requested, I am providing the following testing discount code:

DISCOUNT CODE: #####
Expiration date: ##### Read More

Thank you for your telephone call earlier today.

As discussed on teh phone, as far as we are aware, ab1838 has never been tested with samples from mouse]. All tested species cross-reactivity information is stated on our datasheets, and these...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (U2OS)
Loading amount 25 µg
Specification U2OS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Andrew Cobb

Verified customer

投稿 Mar 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 5 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (acrylamide 9%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Dermal Fibroblast)
Loading amount 10000 cells
Specification Dermal Fibroblast
Treatment XRCC1 or non-targeting siRNA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 17 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Dermal Fibroblast)
Specification Dermal Fibroblast
Fixative Formaldehyde
Permeabilization Yes - Triton
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

投稿 Aug 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (cervix (Hela))
Specification cervix (Hela)
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Alexander Rapp

Verified customer

投稿 Feb 01 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Formaldehyde
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 4%
Username

Mr. Oliver Mortusewicz

Verified customer

投稿 Oct 17 2006

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"