製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to XPG
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from an internal sequence within Human XPG.

  • ポジティブ・コントロール
    • Extracts from K562 cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab64931 was affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa).
ELISA 1/5000.

ターゲット情報

  • 機能
    Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.
  • 関連疾患
    Defects in ERCC5 are the cause of xeroderma pigmentosum complementation group G (XP-G) [MIM:278780]; also known as xeroderma pigmentosum VII (XP7). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-G patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
  • 配列類似性
    Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COFS 3 antibody
    • COFS3 antibody
    • DNA excision repair protein ERCC 5 antibody
    • DNA excision repair protein ERCC-5 antibody
    • DNA excision repair protein ERCC5 antibody
    • DNA repair protein complementing XP G cells antibody
    • DNA repair protein complementing XP-G cells antibody
    • DNA repair protein complementing XPG cells antibody
    • ERCC 5 antibody
    • ERCC5 antibody
    • ERCC5_HUMAN antibody
    • ERCM 2 antibody
    • ERCM2 antibody
    • Excision repair cross complementation group 5 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 5 antibody
    • Excision repair protein antibody
    • OTTHUMP00000064902 antibody
    • UVDR antibody
    • Xeroderma Pigmentosum Complementation Group G antibody
    • Xeroderma pigmentosum complementation group G protein antibody
    • Xeroderma pigmentosum group G complementing protein antibody
    • Xeroderma pigmentosum group G-complementing protein antibody
    • XPG antibody
    • XPG complementing protein antibody
    • XPGC antibody
    see all

画像

  • All lanes : Anti-XPG antibody (ab64931) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from K562 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 133 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Gaddameedhi S  et al. Similar nucleotide excision repair capacity in melanocytes and melanoma cells. Cancer Res 70:4922-30 (2010). WB ; Human . Read more (PubMed: 20501836) »

See 1 Publication for this product

レビューと Q&A

Danke für Ihre Email.
Ich kann Sie da tatsächlich beruhigen. Da wir Produkte in die ganze Welt verschicken, brauchen manche Lieferungen bis zu einer Woche bei unter anderem tropischen Temperaturen.
Deshalb haben wir Testreihen durchgeführt um...

Read More

Vielen Dank für Ihre Anfrage. Das Epitop für diesen XPG-Antikörper wurde leider nicht exakt kartiert. Das Immunogen, das zur Herstellung verwendet wird, ist jedoch zwischen den Aminosäuren x und y lokalisiert und damit sollte der Antikörper nur die ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (GM00637 fibroblast cell line)
Loading amount
100 µg
Specification
GM00637 fibroblast cell line
Gel Running Conditions
Reduced Denaturing (7,5 %)
Blocking step
from another company as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 18°C
Username

Mrs. Annika Schäfer

Verified customer

投稿 May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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