製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to XPF
  • アプリケーション
    適用あり: WB, ELISA, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human XPF.

  • ポジティブ・コントロール
    • Extracts from 293 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab73720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 103 kDa.
ELISA 1/10000.
IP Use at an assay dependent concentration. PubMed: 24726326

ターゲット情報

  • 機能
    Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
  • 関連疾患
    Defects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
    Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly.
  • 配列類似性
    Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA excision repair protein ERCC 4 antibody
    • DNA excision repair protein ERCC-4 antibody
    • DNA excision repair protein ERCC4 antibody
    • DNA repair endonuclease XPF antibody
    • DNA repair protein complementing XP F cells antibody
    • DNA repair protein complementing XP-F cells antibody
    • ERCC 11 antibody
    • ERCC 4 antibody
    • ERCC11 antibody
    • ERCC4 antibody
    • Excision repair complementing defective in Chinese hamster antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 4 antibody
    • excision repair cross-complementation group 4 antibody
    • FANCQ antibody
    • RAD 1 antibody
    • RAD1 antibody
    • Xeroderma pigmentosum complementation group F antibody
    • Xeroderma pigmentosum group F complementing protein antibody
    • Xeroderma pigmentosum group F-complementing protein antibody
    • Xeroderma pigmentosum VI antibody
    • XP, group G antibody
    • XP6 antibody
    • XPF_HUMAN antibody
    see all

画像

  • All lanes : Anti-XPF antibody (ab73720) at 1/500 dilution

    Lane 1 : Extracts from 293 cells
    Lane 2 : Extracts from 293 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 103 kDa
    Observed band size : 103 kDa

参考文献

This product has been referenced in:
  • Hodskinson MR  et al. Mouse SLX4 is a tumor suppressor that stimulates the activity of the nuclease XPF-ERCC1 in DNA crosslink repair. Mol Cell 54:472-84 (2014). WB, IP ; Mouse . Read more (PubMed: 24726326) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (GM00637 fibroblast cell line)
Loading amount
100 µg
Specification
GM00637 fibroblast cell line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
(agent) for 30 minute(s) · Concentration: 5µg/mL · Temperature: 20°C
Username

Mrs. Annika Schäfer

Verified customer

投稿 Apr 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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