製品の概要

  • 製品名Anti-XPD antibody
    XPD 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to XPD
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 34-381 of Human XPD (NP_000391).

  • ポジティブ・コントロール
    • NT2D1, IMR32, U-87 MG and MCF7 cells and whole cell lysates; Human Breast carcinoma tissue.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab111596 is purified by antigen affinity chromatography.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab111596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 87 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: Tris-EDTA buffer pH 8.0
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.
  • 関連疾患Defects in ERCC2 are the cause of xeroderma pigmentosum complementation group D (XP-D) [MIM:278730]; also known as XP group D (XPD). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-D patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
    Defects in ERCC2 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
    Defects in ERCC2 are the cause of cerebro-oculo-facio-skeletal syndrome type 2 (COFS2) [MIM:610756]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • 配列類似性Belongs to the helicase family. RAD3/XPD subfamily.
    Contains 1 helicase ATP-binding domain.
  • 翻訳後修飾ISGylated.
  • 細胞内局在Nucleus. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • TFIIH 80 kDa subunit antibody
    • Basic transcription factor 2 80 kDa subunit antibody
    • BTF2 p80 antibody
    • COFS 2 antibody
    • COFS2 antibody
    • CXPD antibody
    • DNA excision repair protein ERCC 2 antibody
    • DNA excision repair protein ERCC-2 antibody
    • DNA repair protein complementing XP D cells antibody
    • DNA repair protein complementing XP-D cells antibody
    • EM9 antibody
    • ERCC 2 antibody
    • ERCC2 antibody
    • ERCC2_HUMAN antibody
    • Excision repair 2 antibody
    • Excision repair cross complementing rodent repair deficiency complementation antibody
    • Excision repair cross complementing rodent repair deficiency, complementation group 2 antibody
    • MAG antibody
    • MGC102762 antibody
    • MGC126218 antibody
    • MGC126219 antibody
    • OTTHUMP00000045860 antibody
    • OTTHUMP00000045861 antibody
    • OTTHUMP00000045862 antibody
    • OTTHUMP00000045863 antibody
    • TFIIH 80 kDa subunit antibody
    • TFIIH basal transcription factor complex 80 kDa subunit antibody
    • TFIIH Basal Transcription Factor Complex Helicase Subunit antibody
    • TFIIH basal transcription factor complex helicase XPD subunit antibody
    • TFIIH basal transcription factor complex p80 subunit antibody
    • TFIIH p80 antibody
    • TTD antibody
    • Xeroderma pigmentosum complementary group D antibody
    • Xeroderma pigmentosum group D complementing protein antibody
    • Xeroderma pigmentosum group D-complementing protein antibody
    • XPD antibody
    • XPDC antibody
    see all

Anti-XPD antibody 画像

  • Anti-XPD antibody (ab111596) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size : 87 kDa
  • ab111596 at 1/500 dilution staining XPD in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
  • ab111596 at range of 1/100- 1/1000 dilution staining XPD in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.

Anti-XPD antibody (ab111596) 使用論文

ab111596 has not yet been referenced specifically in any publications.

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