• 製品名Anti-XPC antibody [3.26]
    XPC 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3.26] to XPC
  • アプリケーション適用あり: ICC/IF, IHC-P, Flow Cyt, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length human XPC protein expressed in E. coli.

  • ポジティブ・コントロール
    • Raji whole cell lysate, HeLa cell lysate.
  • 特記事項One customer reported cross-reaction of the antibody with p53.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Purified from tissue culture supernatant.
  • ポリ/モノモノクローナル
  • クローン名3.26
  • ミエローマNS1
  • アイソタイプIgG1
  • 研究分野


Our Abpromise guarantee covers the use of ab6264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1-2µl for 106 cells.
WB 1/500 - 1/1000. Detects a band of approximately 113-120 kDa (predicted molecular weight: 105 kDa). Block with 1% BSA instead of milk.


  • 機能Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.
    The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.
  • 関連疾患Defects in XPC are a cause of xeroderma pigmentosum complementation group C (XP-C) [MIM:278720]; also known as xeroderma pigmentosum III (XP3). XP-C is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities.
  • 配列類似性Belongs to the XPC family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated upon UV irradiation; the ubiquitination requires the UV-DDB complex, appears to be reversible and does not serve as a signal for degradation.
  • 細胞内局在Nucleus. Cytoplasm. Omnipresent in the nucleus and consistently associates with and dissociates from DNA in the absence of DNA damage. Continuously shuttles between the cytoplasm and the nucleus, which is impeded by the presence of NER lesions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA repair protein complementing XP C cells antibody
    • DNA repair protein complementing XP-C cells antibody
    • DNA repair protein complementing XPC cells antibody
    • p125 antibody
    • RAD4 antibody
    • Xeroderma pigmentosum complementation group C antibody
    • Xeroderma pigmentosum group C complementing protein antibody
    • Xeroderma pigmentosum group C protein antibody
    • Xeroderma pigmentosum group C-complementing protein antibody
    • Xeroderma pigmentosum group III antibody
    • XP 3 antibody
    • XP C antibody
    • XP group C antibody
    • XP3 antibody
    • Xpc antibody
    • XPC gene antibody
    • XPC_HUMAN antibody
    • XPCC antibody
    see all

Anti-XPC antibody [3.26] 画像

  • Lane 1 : Anti-XPC antibody [3.26] (ab6264) at 1/1000 dilution
    Lane 2 : Anti-XPC antibody [3.26] (ab6264) at 1/500 dilution

    Lane 1 : Raji whole cell extract
    Lane 2 : Raji whole cell extract

    Predicted band size : 105 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
  • Ab6264 staining human XPC in HeLa cells (left), and DAPI staining (right) by immunofluorescence.
  • ab6264 (4µg/ml) staining XPC in human tonsil, using an automated system (DAKO Autostainer Plus). Using this protocol there is weak nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing A431 cells stained with ab6264 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6264, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Predicted band size : 105 kDa
    Western blot image of Human XPC from HeLa whole cell lysate using:
    Lane 1: Rabbit polyclonal ab21078
    Lane 2: Rabbit polyclonal ab21082
    Lane 3: Rabbit monoclonal ab6264

Anti-XPC antibody [3.26] (ab6264) 使用論文

This product has been referenced in:
  • Guillemette S  et al. FANCJ localization by mismatch repair is vital to maintain genomic integrity after UV irradiation. Cancer Res 74:932-44 (2014). Read more (PubMed: 24351291) »
  • Yuan F  et al. Overexpressed DNA polymerase iota regulated by JNK/c-Jun contributes to hypermutagenesis in bladder cancer. PLoS One 8:e69317 (2013). WB . Read more (PubMed: 23922701) »

See all 15 Publications for this product

Product Wall

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided...

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I will be happy to send a replacement but have you considered one of the other XPC antibodies, for instance ab21082? I am worried the monoclonal will give you the same result as before.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

As requested, I have issued a free of charge replacement with the order number **** for the alter...

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Merci pour votre appel de hier ainsi que pour votre patience. Nous nous excusons encore une fois pour la légende manquant pour le ab21078. Nous avons mis ceci à jour maintenant. Les puits correspondent tous à des cellules HeLa, puits nr 1 était fait av...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (fibroblast cell line)
Loading amount 100 µg
Specification fibroblast cell line
Gel Running Conditions Reduced Denaturing (6 %)
Blocking step Blocking buffer from another company as blocking agent for 30 minute(s) · Temperature: 22°C

Mrs. Christiane Kuschal

Verified customer

投稿 Apr 25 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (fibroblast)
Loading amount 40 µg
Specification fibroblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Fabien Gosselet

Verified customer

投稿 Mar 15 2006

In general, we recommend loading 20 µg of the Raji cell lysate per lane for a mini gel. The Raji cell lysate was not stimulated. If you have any more questions, please let me know.

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with this antibody. Yes, ab6264 was tested and characterized using Raji whole cell lysate (there is a Western blot image on the online datasheet for ab6294). It is di...

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Thank you for your enquiry and your patience. The strain that was used was BL21 (DE3). Unfortunately, the vector that was used is proprietary information that we cannot reveal.

The MW of the the human XPG protein is 185 kDa and clone 8H7 binds between human XPG residues Ser 947 and Ala 1165. If you have any more questions, please contact us again.