The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報IHC-P: 1:50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of antigen unmasking fluid for this purpose is recommended. A two hour incubation with Ab10905 is recommended.
WB: 1/5000. Detects a band of approximately 40-42 kDa doubled (predicted molecular weight: 32.8 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
組織特異性Expressed in various cell lines and in skin fibroblasts.
関連疾患Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
配列類似性Belongs to the XPA family.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated by HERC2 leading to degradation by the proteasome.