製品の概要

  • 製品名Anti-XPA antibody [5F12]
    XPA 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5F12] to XPA
  • アプリケーション適用あり: ELISA, WB, Inhibition Assay, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant full length XPA protein (Human)

  • エピトープAmino acids 30-47.
  • ポジティブ・コントロール
    • Crude extract of HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • 精製度Ion Exchange Chromatography
  • ポリ/モノモノクローナル
  • クローン名5F12
  • アイソタイプIgG2b
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab65963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
Inhibition Assay Use at an assay dependent concentration. Inhibition of in vitro excision repair reaction and inhibition of XPA interaction with ERCC1 and TFIIH.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
  • 組織特異性Expressed in various cell lines and in skin fibroblasts.
  • 関連疾患Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
  • 配列類似性Belongs to the XPA family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by HERC2 leading to degradation by the proteasome.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA repair protein complementing XP A cells antibody
    • DNA repair protein complementing XP-A cells antibody
    • DNA repair protein complementing XPA cells antibody
    • Excision repair controlling antibody
    • Xeroderma pigmentosum 1 antibody
    • Xeroderma pigmentosum complementation group A antibody
    • Xeroderma pigmentosum group A complementing protein antibody
    • Xeroderma pigmentosum group A-complementing protein antibody
    • XP 1 antibody
    • XP1 antibody
    • xpa antibody
    • XPA_HUMAN antibody
    • Xpac antibody
    see all

Anti-XPA antibody [5F12] 画像

  • Immunofluorescent staining of human fibroblast cells (GM0637) using ab65963 at a dilution of 1/100. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 (right), fixed after 30 minutes with paraformaldehyde and counter-stained with Hoescht. The top panels show the antibody staining, the middle panels show the Hoescht staining and the bottom panels are a merge of the two images. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-mouse IgG, used at a dilution of 1/5000.

  • Anti-XPA antibody [5F12] (ab65963) at 0.1 µg/ml + Crude extract of HeLa cells at 200 µg

    Predicted band size : 31 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-XPA antibody [5F12] (ab65963) 使用論文

This product has been referenced in:
  • Sousa MM  et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One 8:e55493 (2013). WB ; Human . Read more (PubMed: 23405159) »
  • Herrera M  et al. Differences in repair of DNA cross-links between lymphocytes and epithelial tumor cells from colon cancer patients measured in vitro with the comet assay. Clin Cancer Res 15:5466-72 (2009). WB ; Human . Read more (PubMed: 19690199) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab65963.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"