Anti-XPA 抗体 [5F12] (ab65963)
Key features and details
- Mouse monoclonal [5F12] to XPA
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-XPA antibody [5F12]
XPA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5F12] to XPA -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Recombinant full length XPA protein (Human)
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エピトープ
Amino acids 30-47. -
ポジティブ・コントロール
- WB: HeLa and 293T cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
ポリ/モノ
モノクローナル -
クローン名
5F12 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65963の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa). |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. -
組織特異性
Expressed in various cell lines and in skin fibroblasts. -
関連疾患
Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders. -
配列類似性
Belongs to the XPA family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by HERC2 leading to degradation by the proteasome. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7507 Human
- Entrez Gene: 22590 Mouse
- Omim: 611153 Human
- SwissProt: P23025 Human
- SwissProt: Q64267 Mouse
- Unigene: 654364 Human
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別名
- DNA repair protein complementing XP A cells antibody
- DNA repair protein complementing XP-A cells antibody
- DNA repair protein complementing XPA cells antibody
see all
画像
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All lanes : Anti-XPA antibody [5F12] (ab65963) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : XPA knockout HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab65963 observed at 38 kDa. Red - loading control, ab52866 observed at 50 kDa.
ab65963 Anti-XPA antibody [5F12] was shown to specifically react with XPA in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264663 (knockout cell lysate ab258764) was used. Wild-type and XPA knockout samples were subjected to SDS-PAGE. ab65963 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (ab52866) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Immunofluorescent staining of human fibroblast cells (GM0637) using ab65963 at a dilution of 1/100. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 (right), fixed after 30 minutes with paraformaldehyde and counter-stained with Hoescht. The top panels show the antibody staining, the middle panels show the Hoescht staining and the bottom panels are a merge of the two images. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-mouse IgG, used at a dilution of 1/5000.
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Anti-XPA antibody [5F12] (ab65963) at 0.1 µg/ml + Crude extract of HeLa cells at 200 µg
Predicted band size: 31 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
All lanes : Anti-XPA antibody [5F12] (ab65963) at 1/2000 dilution
Lane 1 : HeLa cell extract (XPA wild type)
Lane 2 : XP12ROSV cell extract (XPA deficient)
Predicted band size: 31 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab65963 は 8 報の論文で使用されています。
- Shorrocks AK et al. The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks. Nat Commun 12:585 (2021). PubMed: 33500419
- Snow JA et al. ß-HPV 8E6 Attenuates ATM and ATR Signaling in Response to UV Damage. Pathogens 8:N/A (2019). PubMed: 31779191
- Holcomb N et al. Inorganic arsenic inhibits the nucleotide excision repair pathway and reduces the expression of XPC. DNA Repair (Amst) 52:70-80 (2017). PubMed: 28237621
- Shafirovich V et al. Base and Nucleotide Excision Repair of Oxidatively Generated Guanine Lesions in DNA. J Biol Chem 291:5309-19 (2016). PubMed: 26733197
- Starczewska E et al. Targeting DNA repair with aphidicolin sensitizes primary chronic lymphocytic leukemia cells to purine analogs. Oncotarget 7:38367-38379 (2016). PubMed: 27223263
- Sousa MM et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One 8:e55493 (2013). WB ; Human . PubMed: 23405159
- Herrera M et al. Differences in repair of DNA cross-links between lymphocytes and epithelial tumor cells from colon cancer patients measured in vitro with the comet assay. Clin Cancer Res 15:5466-72 (2009). WB ; Human . PubMed: 19690199
- Telen MJ et al. A blood group-related polymorphism of CD44 abolishes a hyaluronan-binding consensus sequence without preventing hyaluronan binding. J Biol Chem 271:7147-53 (1996). PubMed: 8636151